Invention relates to a process for preparing Nanoparticles from ZnO is provided, the process involves co-precipitation method. 0.02 M aqueous solution of zinc acetate di-hydrate is put into 100 ml of distilled water under vigorous stirring. After 10 min stirring, 3mL-5ml of extract prepared from the prawn is added the above solution. After addition of extract, 2.0 M aqueous sodium hydroxide solution is introduced into the above aqueous solution drop wise, resulting in a white aqueous solution at pH 12, which is then placed on magnetic stirrer for 2 hr. The precipitate is then taken out and washed repeatedly with distilled water followed by ethanol to remove the impurities for the final products. Then a white powder is obtained after drying at 60 °C in vacuum oven overnight. In another aspect of the invention Characterization of ZnO is done by Transmission electron microscopy (TEM) and Scanning electron microscopy (SEM) and UV-Vis Spectrophotometer. Further, the ZnO nanoparticles prepared are studied for the Antibacterial activity of synthesized Zn Materials used for antimicrobial activity of zinc oxide nanoparticles are Nutrient agar slants, petriplates, Cork borer, zinc oxide nanoparticle sample, Salmonella typhi, salmonella paratyphi A and Salmonella paratyphi B. Agar diffusion method is used for antimicrobial activity of zinc oxide nanoparticles.
Status of Patent: Indian Patent Pending No.2422/MUM/2013