Production of Enzyme L-Glutaminase From Bacteria Present in Gut Region of Penaeus Monodon

Production of Enzyme L-Glutaminase From Bacteria Present in Gut Region of Penaeus Monodon

Invention relates to black tiger Penaeus monodon is selected from the local market, the gut was homogenised with sterile 1 % peptone water for enrichment, these enriched gut pieces were plated on nutrient agar, and incubated. Further, microbes isolated are screened for the glutaminase production which is done on modified minimal agar medium which is containing L- glutamine as a sole source of nitrogen & phenol red as a pH indicator. In an other aspect of the invention the microbes isolated from the gut region of the prawns is identified as bacterium Pseudomorias aeruginosa. In one of the aspects of the invention, screening M9 medium is used containing L-glutamine as a sole source of nitrogen & phenol red as a pH indicator so that if L-glutaminase producer is present, it breaks the amide bond of L-glutamine and due to this slight alkaline condition, colony becomes pink & non-producer colonies are pale yellow. Further, production & purification is done. Purified enzyme L-glutaminase is checked for its cytotoxic effect on normal cells and then on cancerous cell lines.
Status of Patent: Indian Patent Pending No.468/MUM/2013